Biochemistry

Biochemistry is the study of the chemical processes occurring in living matter. It majorly focus on the role, function, and structure of biomolecules. In general it deals with enzymes, carbohydrates, amino acids, proteins, fats, hormones, DNA, RNA, etc.

Biochemistry of Urine or Urine analysis or Urinalysis or Urine-R&M(routine and microscopy)

Urine is an excretory product of the body and presence of certain substances in urine reflects the metabolic state of the body. Composition 95% of volume of normal urine is due to water. Others:- Organic components – urea, urobilinogen, uric acid, amino acids, creatinine, metabolities of hormones. Inorganic components – Cations:- Na+, K+, Ca2+, NH4+ …

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Amino acids and Proteins

Living systems are made up of Proteins .They are the dehydration polymers of amino acids.Each amino acid residue is joined by a peptide linkage to form proteins. Proteins are the molecular instruments in which genetic information is expressed, Hormones, Antibodies,transporters, the lens protein, the architectural framework of our tissues and a myriad of substances having distinct …

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Enzymes

Body proteins perform a large number of functions. One such unique function is, they act as biological catalysts (Enzymes) .They are responsible for highly complex reactions. They direct the metabolic events and exhibit specificity toward substrates, regulate the entire metabolism.Thus, they play key role in the degradation and synthesis of nutrients, biomolecules etc. The most …

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Vectors

A vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated or expressed. Cloning vector is a small DNA molecule capable of self-replication inside the host cell. Cloning vector is used for replicating donor DNA fragment within host cell. Vectors can replicate …

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Ramchandran plot / Ramachandran diagram / Ø, ψ plot

Developed by: G.N. Ramachandran in 1963 + and – + and + – and – 0 White regions: sterically disallowed for all amino acids except glycine Red regions: allowed regions namely the alpha-helical and beta-helical conformations Yellow areas: outer limit Exception: Glycine can lie anywhere Oneplus Nord CE4 (Celadon Marble, 8GB RAM, 128GB Storage) 4.2 …

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Structure of Glyceraldehyde-3 phosphate dehydrogenase

Active site: Cys 149, His 176 adjacent to NAD Glyceraldehydes 3 phosphate + Pi + NAD+   –gives–       1,3-Bisphosphoglycerate + NADH + H+ iQOO Neo9 Pro 5G (Fiery Red, 8GB RAM, 256GB Storage) | Snapdragon 8 Gen 2 Processor | Supercomputing Chip Q1 | Flagship Level Sony IMX920 Camera 4.3 out of 5 stars(1330) ₹36,999.00 (as of …

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Glycolysis or Embden-Meyerhof pathway (EM-pathway)

Derived from: Glykys – sweet Lysis – breakdown/loosing That is glycolysis means: loosing or splitting of Glucose Produces energy: -60 KJ/mol. Occurs: almost in every living cell Glycolysis takes place in both aerobic and anaerobic organisms Site: Cytosol Glycolysis is the central pathway for the glucose catabolism in which glucose (6-carbon compound) is converted into …

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Difference between Differential Leukocyte Count (DLC) and Total Leukocyte Count (TLC)

DLC means the count of individual WBC cells. Thus helps in knowing which specific cell type is increased in blood While TLC means the total no. of WBC i.e., helps to know the total no. of white blood cell count : Includes: Neutropils Lymphocytes Monocytes Eosinophils Basophils Oneplus Nord CE4 (Celadon Marble, 8GB RAM, 128GB …

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Reverse blood grouping

Introduction:                       ABO grouping is performed in two ways: Forward grouping/cell grouping: red blood cells are tested for A and B antigens using known anti-A and anti-B sera. (Identification of ABO antigens on red blood cells) Reverse grouping/serum grouping: serum is tested for anti-A and anti-B antibodies using known A and B red cells. (Identification …

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